In this research, hydroxyapatite composite with two reinforcements alumina and zirconia has been used as a coating on titanium dental implants for the first time. Two kinds of titanium implants with different percentages of impurities were chosen and after cleaning, they were coated with hydroxyapatite alumina-zirconia sol. The method of coating was sol-gel that coated implants by dip-coating and spin-coating. The use of sol-gel method was for the reason that it is a cheap coating method and it can coat all samples. By comparing the samples, it was found that in dip-coating method, cracks are less and the morphology is more homogeneous. Although spin-coated samples were homogeneous too, but they had more cracks. coating processes were done in three stages and between these stages, in second stage, coatings were the best in having less cracks and the best homogeneity. It was found that implants with less impurity had more homogeneous coatings. Increasing aging and calcination temperature of coatings had a great effect on the quality of coatings. The aim of this research was to investigate these coatings for coating titanium dental implants. Because implantation is an expensive operation, we used cheap raw materials for the coating of implants.

Background: Studying protein-protein and protein-DNA interactions are prerequisites for the identification of function and mechanistic role of various proteins in the cell. Protocols for analyzing DNA-based Protein-Protein and Protein-DNA interactions are complicated and need to be simplified for efficient tracking of binding capabilities of various proteins to specific DNA molecules. Here, we demonstrated a simple yet efficient protocol for the identification of DNA coating-based Protein-DNA interaction using antibodymediated immunodetection. Methods: Briefly, we have coated specific DNA in the microtiter plate followed by incubating with protein lysate. Specific protein-DNA and/or protein-protein bind with DNA interactions are identified using specific fluorophore-conjugated antibodies. Antibodies are used to detect a protein that is bound to the DNA. Results: Fluorescent-based detection identifies the specific interaction between Protein-DNA with respect to coated DNA fragments. The protocol uses indirect conjugated antibodies and hence the technique is sensitive for effective identification of Protein-DNA interactions. Conclusions: Based on the results we conclude that the demonstrated protocol is simple, efficient and sensitive for identification of Protein-DNA interactions.

In this paper, the electrical transport of graphene and silicene nanostructures connected to two semi-infinite graphene and silicene electrodes have been investigated. In these structures, we created nanopores and passed the DNA molecule through the nanopore. Using the Green's Function method and Tight binding approximation, two types of systems with zigzag edges in graphene and silicene have been investigated. By connecting the nanoribbons to the metal electrodes, the created current passes from the nanopore and DNA, from the left to the right. This electric current flow increases the system's sensitivity to the organic DNA bases. We have introduced a new scheme for DNA sequencing.

Objective: Traditional methods for storing microbial DNA samples use the low-temperatures of 4oC, -20oC or -80oC. The DBC Card is a new method of storing microbial DNA at room temperature. This study evaluates the stability and determine the best storing method of Bacterial DNA on the DBC card.Methods: In this study, we used a pair of primers from the conserved domain of 16srRNA designed to identify Escherichia coli. Four different types of samples we prepared: i) bacterial suspension, ii) bacterial DNA extracted by the phenol-chloroform method, iii) bacterial lysate with lysis buffer and iv) bacterial DNA produced by the boiling method. All four samples were spotted on separate DBC cards and dried at room temperature. After periods of 3, 5 and 7 months, Escherichia coli samples were checked for DNA stability with two molecular techniques, conventional PCR with 1, 2 and 3 disks as a source of bacterial DNA (1 mm diameter) and Real-Time PCR.Results: Data showed that DNA stability was maintained after 7 months on a DBC disk, even using only one disk as a DNA source. The bacterial suspension was the best method for long-term storage of Escherichia coli DNA on the DBC Card.Conclusion: In traditional methods for storing sample, DNA quality reduces after freezing and thawing. However in the DBC method, DNA quality was maintained for a long duration. Advantages of the DBC method are easy sample handing, low cost, faster extraction, and reduced individual and environmental contamination.